Učni načrt predmeta

Predmet:

Sintezna biologija in biosenzorika

Course:

Synthetic Biology and Biosensing

Študijski program in stopnja / Study programme and level	Študijska smer / Study field	Letnik / Academic year	Semester / Semester
Senzorske tehnologije, 3. stopnja	/	1	1
Sensor technologies, 3rd cycle	/	1	1

Vrsta predmeta / Course type

Izbirni / Elective

Univerzitetna koda predmeta / University course code:

ST3-541

Predavanja Lectures	Seminar Seminar	Vaje Tutorial	Klinične vaje work	Druge oblike študija	Samost. delo Individ. work	ECTS
10	10			30	250	10

*Navedena porazdelitev ur velja, če je vpisanih vsaj 15 študentov. Drugače se obseg izvedbe kontaktnih ur sorazmerno zmanjša in prenese v samostojno delo. / This distribution of hours is valid if at least 15 students are enrolled. Otherwise the contact hours are linearly reduced and transfered to individual work.

Nosilec predmeta / Course leader:

prof. dr. Tamara Lah Turnšek

Sodelavci / Lecturers:

prof. dr. Henning Ulrich

Jeziki / Languages:

Predavanja / Lectures:

Slovenski ali angleški / Slovene or English

Vaje / Tutorial:

Pogoji za vključitev v delo oz. za opravljanje študijskih obveznosti:

Prerequisites:

Zaključen študij druge stopnje ustrezne (naravoslovne ali tehniške) smeri ali zaključen študij drugih smeri z dokazanim poznavanjem osnov področja predmeta (pisna dokazila, pogovor).

Completed second level studies in natural sciences or engineering or completed second level studies in other fields with proven knowledge of fundamentals in the field of this course (certificates, interview).

Vsebina:

Content (Syllabus outline):

- Uvod: koncept biosenzorjev: biološki mehanizmi, zaznavni in prevodni sistemi (več predavateljev).
- Sintezna biologija: uvajanje in priprave novih umetnih bioloških poti, organizmov ali novo preurejanje iz obstoječih naravnih bioloških sistemov za biotehnološke namene in v diagnostiki ter zdravljenju.
- Uporaba DNA in RNA inženiringa za namene ustvarjanja osnovnih bioloških komponent.
- Inženiring bioloških snovi osnovanih na regulatornih zankah RNA.
- RNA molekule za programiranje genskega izražanja in odziv majhnih molekul za uporabo kot biosenzorji.
- RNA in DNA aptameri v biosenzoriki: principi in uporaba v primerjavi s klasičnimi testi uporabe (protitelesa).
- Novi pristopi v zaznavanju metabolitov in proteinov v živih celicah z RNA aptameri.
- Aptameri za zaznavanje matičnih in rakavih celic in patogenih organizmov.
- On-chip proteinske mreže za multipleksna SPRI merjenja v raziskavah in klinični uporabi.
- Alosterični ribozimi in deoksi ribozimi kot biosenzorji.
- Biosenzorji na osnovi RNA in DNA v varovanju okolja in varne hrane.
- Biosensorji v raziskavah raka: tehnologije biomarkerjev.

- Introduction: Concept of biosensors: Biological mechanisms, detection and translation systems (more authors).
- Synthetic biology: Introducing the concept and construction of novel artificial biological pathways, organisms, or the redesign of existing natural biological systems for purposes of biotechnology, diagnostics and therapy.
- Application of DNA and RNA engineering principles to design the fundamental biological components.
- Engineering of biological substances, based on RNA regulatory loops.
- RNA molecules for programming gene expression in response to small molecules. Applications for biosensorics.
- RNA and DNA aptamers for biosensorics: Principles and applications vs. classical (antibody-based) assays.
- New approaches for sensing metabolites and proteins in live cells using RNA aptamers.
- Aptamers for detection of stem, cancer cells and pathogenic organisms.
- On-chip protein microarray for multiplexed SPRI biosensing measurements in research and clinical application.
- Allosteric ribozymes and deoxy-ribozymes as biosensors.
- RNA- and DNA-derived biosensors in environmental protection and food safety.
- Biosensors in cancer research: Biomarkers technologies.

Temeljna literatura in viri / Readings:

- Arugula MA, Zhang Y, Simonian A. Biosensors as 21st Century technology for detecting Genetically Modified Organisms in food and feed. Anal Chem. 2013 Oct 2. [Epub ahead of print
- Bacchus W, Aubel D, Fussenegger M. Biomedically relevant circuit-design strategies in mammalian synthetic biology. Mol Syst Biol. 2013 Sep 24;9:691.
- Bacchus W, Fussenegger M. Engineering of synthetic intercellular communication systems. Metab Eng. 2013 Mar;16:33-41. doi: 10.1016/j.ymben.2012.12.001.
- Benenson Y. Synthetic biology with RNA: progress report. Curr Opin Chem Biol. 2012 Aug;16(3-4):278-84.
- Breaker RR. Engineered allosteric ribozymes as biosensor components. Curr Opin Biotechnol. 2002 Feb;13(1):31-9. Review.
- Chen Y, Nakamoto K, Niwa O, Corn RM. On-chip synthesis of RNA aptamer microarrays for multiplexed protein biosensing with SPR imaging measurements. Langmuir. 2012 Jun 5;28(22):8281-5.
- Liu J, Cao Z, Lu Y. Functional nucleic acid sensors. Chem Rev. 2009 May;109(5):1948-98.
- McKeague M, Giamberardino A, DeRosa MC. Advances in Aptamer-Based Biosensors for Food Safety. In: "Environmental Biosensors", book edited by Vernon Somerset, ISBN 978-953-307-486-3, InTech, 2011
- Paige JS, Nguyen-Duc T, Song W, Jaffrey SR. Fluorescence imaging of cellular metabolites with RNA. Science. 2012 Mar 9;335(6073):1194.
- Robert S. Marks, Christopher R. Lowe, David C. Cullen, Howard H. Weetall, Isao Karube. Handbook of Biosensors and Biochips. Wiley 2007, ISBN: 978-0-470-01905-4.
- Šmuc T, Ahn IY, Ulrich H. Nucleic acid aptamers as high affinity ligands in biotechnology and biosensorics. J Pharm Biomed Anal. 2013 Jul-Aug;81-82:210-7
- Strehlitz B, Reinemann C, Linkorn S, Stoltenburg R. Aptamers for pharmaceuticals and their application in environmental analytics. Bioanal Rev. 2012 Mar;4(1):1-30.
- Ulrich H, Wrenger C. Disease-specific biomarker discovery by aptamers. Cytometry A. 2009 Sep;75(9):727-33
- Win MN, Liang JC, Smolke CD. Frameworks for programming biological function through RNA parts and devices. Chem Biol. 2009 Mar 27;16(3):298-310.
- Zimbres FM, Tárnok A, Ulrich H, Wrenger C. Aptamers: Novel Molecules as Diagnostic Markers in Bacterial and Viral Infections? Biomed Res Int. 2013;2013:731516.

Cilji in kompetence:

Objectives and competences:

- Razumeti biologijo RNA in DNA ter sodobne tehnologije biosenzorjev na njeni osnovi.
- Sposobnost načrtovati in vrednotiti pristope za razvoj biosenzorjev.
- Razumeti, kako lahko umetni tokokrog (v sintezni biologiji) uporabimo za biosenzorje.
- Razumeti interdisciplinarni pristop.

Kompetence:
Znati samostojno uporabljati interedisciplinarne pristope pri razvoju in pripravi biosenzorjev na osnovi nukleinskih kislin.

- Comprehension of RNA and DNA biology and state-of-the art techniques for biosensors.
- Ability to design and evaluate approaches for biosensor development.
- How artificial circuits (synthetic biology) can be used for biosensor approaches.
- Understanding of the importance of interdisciplinary approaches.

Competence
Independent use of tolls to develop the interdisciplinary approach in preparation of nuclear acids base biosensors.

Predvideni študijski rezultati:

Intendeded learning outcomes:

Znanje in razumevanje:
- Interdisciplinarnih pristopov.
- Osvojeno znanje predavanj.
- Ustvarjanje neodvisnega mišljenja v raziskavah.

Knowledge and understanding of:
- Interdisciplinary approaches.
- Knowledge on taught subjects.
- Formation of independent researcher´s skills.

Metode poučevanja in učenja:

Learning and teaching methods:

- Predavanja profesorjev.
- Interaktivno obravnavanje novih objav iz izbranih področij raziskav s profesorjem.
- Seminar študentov.
- Praktična projektna naloga s področja razvoja biosenzorja.

- Lectures by the professor.
- Discussion groups where student read selected papers and discuss their content with the professor.
- Students' seminars.
- Practical project in the biosensors field.

Načini ocenjevanja:

Delež v % / Weight in %

Assesment:

Ustni izpit

Oral exam

Seminarska / projektna naloga

Seminar / Project for biosensor-development

Reference nosilca / Lecturer's references:

1.	TORSVIK, Anja, PRIMON, Monika, LAH TURNŠEK, Tamara, MOTALN, Helena. Spontaneous malignant transformation of human mesenchymal stem cells reflects cross-contamination: putting the research field on track - letter. Cancer res. (Baltimore), 2010, 70, 15, 6393-6396.
2.	MOTALN, Helena, SCHICHOR, Christian, LAH TURNŠEK, Tamara. Human mesenchymal stem cells and their use in cell-based therapies. Cancer (Print), 2010, vol. 116, no. 11,. 2519-2530.
3.	KOLOŠA, Katja, LESKOVŠEK, Aleš, RAJAR, Teja, LAH TURNŠEK, Tamara. Fast assay to predict multipotent mesenchymal stromal cell replicative senescence dynamics. Biotechniques. [Print ed.]. 2022, vol. 72, no. 3, str. 1-10, ilustr. ISSN 0736-6205. https://www.future-science.com/doi/10.2144/btn-2021-0087, DOI: 10.2144/btn-2021-0087
4.	LAH TURNŠEK, Tamara, NOVAK, Metka, PENA ALMIDON, Milagros A., MARINELLI, Oliviero, ŽVAR BAŠKOVIČ, Barbara, MAJC, Bernarda, MLINAR, Mateja, BOŠNJAK, Roman, BREZNIK, Barbara, ZOMER, Roby, NABISSI, Massimo. Cannabigerol is a potential therapeutic agent in a novel combined therapy for glioblastoma. Cells. 4 Feb. 2021, vol. 10, [article] 340, str. 1-21, ilustr. ISSN 2073-4409. https://www.mdpi.com/2073-4409/10/2/340, DOI: 10.3390/cells10020340
5.	NEVES OLIVEIRA, Mona das, BREZNIK, Barbara, PILLAT, Micheli M., PEREIRA, Ricardo L., ULRICH, Henning, LAH TURNŠEK, Tamara. Kinins in glioblastoma microenvironment. Cancer microenvironment. 2019, vol. 12, str. 77-94. ISSN 1875-2292. DOI: 10.1007/s12307-019-00229-x