Učni načrt predmeta

Predmet:
Celični senzorji v toksikologiji
Course:
Whole-cell Biosensors in Toxicology
Študijski program in stopnja /
Study programme and level		 Študijska smer /
Study field		 Letnik /
Academic year		 Semester /
Semester
Senzorske tehnologije, 3. stopnja / 1 1
Sensor technologies, 3rd cycle / 1 1
Vrsta predmeta / Course type
Izbirni / Elective
Univerzitetna koda predmeta / University course code:
ST3-542
Predavanja
Lectures		 Seminar
Seminar		 Vaje
Tutorial		 Klinične vaje
work		 Druge oblike
študija		 Samost. delo
Individ. work		 ECTS
5 5 15 125 5

*Navedena porazdelitev ur velja, če je vpisanih vsaj 15 študentov. Drugače se obseg izvedbe kontaktnih ur sorazmerno zmanjša in prenese v samostojno delo. / This distribution of hours is valid if at least 15 students are enrolled. Otherwise the contact hours are linearly reduced and transfered to individual work.

Nosilec predmeta / Course leader:
prof. dr. Bojana Žegura
Sodelavci / Lecturers:
Jeziki / Languages:
Predavanja / Lectures:
Slovenščina, angleščina / Slovenian, English
Vaje / Tutorial:
Pogoji za vključitev v delo oz. za opravljanje študijskih obveznosti:
Prerequisites:

Zaključen študij druge stopnje ustrezne (naravoslovne ali tehniške) smeri ali zaključen študij drugih smeri z dokazanim poznavanjem osnov področja predmeta (pisna dokazila, pogovor).

Completed second-cycle studies in natural sciences or engineering or completed second-cycle studies in other fields with proven knowledge of fundamentals in the field of this course (certificates, interview).

Vsebina:
Content (Syllabus outline):

- Principi in vrste celičnih biosenzorjev.
- Področja aplikacij celičnih biosenzorjev v toksikologiji in ekotoksikologiji.
- Konstrukcija celičnih biosenzorjev glede na namen uporabe (izbira ustreznih odzivnih celičnih elementov, načini zaznavanja odzivov, občutljivost in specifičnost za zaznavanje določenega specifičnega toksičnega učinka ali analita).
- Praktični primeri uporabe celičnih biosenzorjev v (eko)toksikologiji.
- Delo na primerih glede na doktorsko nalogo kandidata.

- Principles and types of whole-cell biosensors.
- Fields of application of whole-cell biosensors in toxicology and ecotoxicology.
- Construction of whole-cell biosensors related to the intended application (selection of relevant responsive cell elements, detection of responses, sensitivity and specificity for selection of specific toxic effect or analyte).
- Case studies of the application of whole-cell based biosensors in (eco)toxicology.
- Case studies related to the PhD study of the candidate.

Temeljna literatura in viri / Readings:

Knjiga / Book:
- Biosensors and Environmental Health, 2012 VR. Preedy, V. Patel (eds), Taylor&Francis group, CRC Press.
- Cell based biosensors: Priciples and applications, 2010. P.Wang, Q. Liu (eds) Artech House, Boston/London, Norwood, MA, USA.

Članki / Journal articles:
- Blagus, T, Žager Marciuš, V, Čemažar, M, Serša, Gr, Kamenšek, U, Žegura, B, Nunić, J, Filipič, M. (2014) The cell-based biosensor system HepG2CDKN1A-DsRed for rapid and simple detection of genotoxic agents. Biosensors & bioelectronics, 2014, 61, 102-111
- Palmer, A.E., Qin, Y., Park, J.G., McCombs, J.E., 2011. Design and application of genetically encoded biosensors. Trends in Biotechnology 29, 144-152.
- Zhong, J.-J., Gu, M., Mitchell, R., Kim, B., 2004. Whole-Cell-Based Biosensors for Environmental Biomonitoring and Application, In Biomanufacturing. pp. 269-305. Springer Berlin Heidelberg.
- Park, M., Tsai, S.L., Chen, W., 2013. Microbial Biosensors: Engineered Microorganisms as the Sensing Machinery. Sensors 13, 5777-5795.
- Gupta, N. et al., 2019. Cell-based biosensors: Recent trends, challenges and future perspectives. Biosensors and Bioelectronics, 141, 15, 2019.
- Tian, F et al., Principles and applications of green fluorescent protein-based biosensors: a mini-review. Analyst, 2023, 148, 2882.

Cilji in kompetence:
Objectives and competences:

Cilji:
- razumevanje stanja razvoja in uporabe celičnih biosenzorjev,
- osvojiti znanja in spretnosti za oblikovanje novih celičnih biosenzorjev na področju toksikologije in ekotoksikologije,
- razvoj in/ali uporaba celičnih biosenzorjev na konkretnih primerih povezanih z usmeritvijo študentovega doktorskega dela.

Objectives:
- understanding of current state of the art in the field of development and applications of whole-cell biosensors,
- to obtain knowledge and skills for design of new whole-cell biosensor in the fields of toxicology and ecotoxicology,
- development and/or application of whole-cell biosensors on specific cases related to directions of students PhD thesis.

Predvideni študijski rezultati:
Intendeded learning outcomes:

- Sposobnost združevanja znanj s področij celične fiziologije, biokemije, molekularne biologije, kemije in sodobnih merilnih tehnik.
- Načrtovanje, konstrukcija in uporabo celičnih biosenzorjev za zaznavanje specifičnih toksičnih učinkov ter za okoljski biomonitoring.
- Samostojno reševanje problemov, povezanih s tematiko doktorske disertacije, ki se nanaša na vsebino predmeta ter povezovanje interdisciplinarnih vsebin.

- Ability of the integration of knowledge in the fields of cell physiology, biochemistry, molecular biology, chemistry and modern measurement techniques.
- Design, construction and use of whole-cell biosensors for detecting specific toxic effects and for environmental biomonitoring.
- Independent solving of the problems related to the topic of the doctoral dissertation, which refer to the content of the course and the integration of interdisciplinary content.

Metode poučevanja in učenja:
Learning and teaching methods:

Prek interaktivne komunikacije med profesorjem in študentom, v obliki diskusij in študentovega samostojnega dela. Reševanje problemov povezanih s potrebami po razvoju novih in/ali uporabo obstoječih celičnih biosenzorjev.

Via interactive communication between professor and student, in form of discussions and individual work. Case studies related to the need for the development and/or application of existing whole-cell biosensors.

Načini ocenjevanja:
Delež v % / Weight in %
Assesment:
Seminar
50 %
Seminar
Reševanje konkretnega primera
50 %
Case study
Reference nosilca / Lecturer's references:
1. ŠTAMPAR, Martina, ŽABKAR, Sonja, FILIPIČ, Metka, ŽEGURA, Bojana. HepG2 spheroids as a biosensor-like cell-based system for (geno)toxicity assessment. Chemosphere. [Print ed.]. 2022, vol. 291, pt. 1, str. 1-11, ilustr. ISSN 0045-6535. https://www.sciencedirect.com/science/article/pii/S004565352103277X#!, DOI: 10.1016/j.chemosphere.2021.132805
2. SENDRA, Marta, ŠTAMPAR, Martina, FRAS, Katarina, NOVOA, Beatriz, FIGUERAS, Antonio, ŽEGURA, Bojana. Adverse (geno)toxic effects of bisphenol A and its analogues in hepatic 3D cell model. Environment international. [Print ed.]. Jan. 2023, vol. 171, str. [1]-19,
3. COLLINS, Andrew, MØLLER, Peter, GAJSKI, Goran, VODENKOVÁ, Soňa, ABDULWAHED, Abdulhadi, ANDERSON, Diana, BANKOGLU, Ezgi Eyluel, BONASSI, Stefano, BOUTET-ROBINET, Elisa, BRUNBORG, Gunnar, NOVAK, Matjaž, ŽEGURA, Bojana, et al. Measuring DNA modifications with the comet assay : a compendium of protocols. Nature protocols. [Online ed.]. 2023, vol. 18, str. 929-989, ilustr. ISSN 1750-2799.
4. ŠTERN, Alja, NOVAK, Matjaž, KOLOŠA, Katja, TRONTELJ, Jurij, ŽABKAR, Sonja, ŠENTJURC, Tjaša, FILIPIČ, Metka, ŽEGURA, Bojana. Exploring the safety of cannabidiol (CBD) : a comprehensive in vitro evaluation of the genotoxic and mutagenic potential of a CBD isolate and extract from Cannabis sativa L. Biomedicine & pharmacotherapy. [Online ed.]. 2024, 177,
5. SANZ-SAGUÉ, Beatriz, SÁENZ-HERNÁNDEZ, Amaia, ŽEGURA, Bojana, ŠTERN, Alja, KOLOŠA, Katja, ROZMAN, Iza, et al. Genotoxicity and heating performance of VxFe3-xO4 nanoparticles in health applications. Chemico-biological interactions. [Print ed.]. 2024, 394, 110977, [1]-13,